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Subscribe now. Shape Created with Sketch. Travel destinations: 52 places to go in Show all Puerto Rico is representative of the many fragile places around the globe right now: the islands facing a future of sea level rise and extreme weather. The arctic spots where winter itself is under threat.

The cities where a combination of climate change and bad planning has resulted in devastation. That is why Puerto Rico earned the number one spot on our annual list of 52 places to visit in the coming year. The island and the other beautiful places at risk raise an urgent question: do we owe something to the places that make us happy? The idea that as visitors we should not cause harm and should seek out authentic experiences that get us deep into the local culture. Perhaps it would not be such a stretch to redefine the relationship between leisure travellers and their dream destinations.

Mireya Navarro. At the height of the Vijayanagar empire in the 16th century, Hampi thrived as one of the largest and richest cities in the world. Its architectural legacy lives on in the southwestern state of Karnataka with over 1, well-preserved stone monuments, including Hindu temples, forts and palaces. Spread over 16 miles near the banks of the Tungabhadra river, and surrounded by a sea of granite boulders, the Unesco world heritage site has been notoriously difficult to reach, until now.

TruJet recently began daily direct flights from Hyderabad and Bangalore to Ballari, a mile drive from Hampi. The outfitters Black Tomato and Remote Lands now offer journeys in the region, from guided archaeological tours to rock climbing and river jaunts in basket boats. Nora Walsh. Long known for drawing movie stars and millionaires to its resorts, Santa Barbara is now a foodie magnet. Acclaimed chef Jesse Singh oversees Bibi Ji, an edgy Indian restaurant — try the uni biryani — with a wine list curated by noted sommelier Rajat Parr.


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Frequency and Melville feature modern furnishings and party-ready playlists; vinyl rules at Sanguis, a winery run by drummers. Sheila Marikar. Cayuga Hospitality recently opened Isla Palenque in the Gulf of Chiriqui, with eight casitas and one villa on a lush acre island. Besides offering access to seven beaches, mangrove kayaking and whale-watching, the resort grows some of its own food, has furniture made from fallen trees and maintains a no-plastics policy, including subbing papaya shoots for straws.

The solar-powered, nine-bungalow lodge offers sport fishing and scuba diving, and composts food waste and recycles water for irrigation. Elaine Glusac. And its museums are decidedly world class, especially since the renovation and reopening of the Lenbachhaus museum in , with its unmatched collection of the German artists known as the Blue Rider school. But perhaps the best argument for visiting Munich right now is the Bavarian State Opera, which has emerged as one of the most exciting opera houses in Europe.

The reason? Petrenko has just two more years remaining on his contract as music director at the opera. Stuart Emmrich. To get there, visitors used to have to catch a charter flight from Tel Aviv or brave the dusty drive through the Negev desert. But with the opening early this year of Ramon Airport, set in the Timna Valley and capable of handling 4 million international transit passengers a year, the world will finally get a direct route — with nonstops from Munich and Frankfurt on Lufthansa, and budget carriers flying in from Prague, London and across Europe.

Debra Kamin. The Setouchi region will host the Setouchi Triennale , a major art fair held in three seasonal instalments. One hour south via ferry or the Shinkansen bullet train, the Hiroshima Peace Memorial Museum reopens this spring after an eight-year refurbishment. For those seeking more sybaritic forms of transport, the Guntu — more a minimalist floating ryokan than a cruise ship — with 19 walnut-clad rooms and open-air cypress soaking baths.

In , Setouchi Sea Planes will expand its scenic flights to several smaller islands and towns via Kodiak s. Adam H Graham. Today, the city is turning its most famous natural asset into an artistic one. Wildly innovative buildings have sprouted on its shores, including the Utzon Centre, designed by Jorn Utzon, the architect of the Sydney Opera House — its new exhibition series on inspiring Nordic architects, runs through May. The curvilinear concert hall Musikkens Hus was recently followed by the vibrant Aalborg Street Food market; the pedestrian and cycling Culture Bridge; and the undulating Vestre Fjordpark, with an open-air swimming pool that meets the sea.

Nordkraft, a power plant that was converted into a cultural hub, is celebrating its 10th anniversary with events in September. Annelisa Sorensen. In the nippy Atlantic Ocean a four-hour flight from the US, the subtropical volcanic islands of the Azores, complete with Unesco world heritage sites and biospheres, await discovery. Mystical green lushness, oversize volcanic craters now turned into lakes, steaming natural hot springs that puff out from the earth, blue hydrangeas by the thousands and the only coffee growers in Europe distinguish the island chain.

Daniel Scheffler. The ice caves that emerge from the winds and waves that pound the north shore of Lake Superior have always been somewhat ephemeral.

Five Best: Christopher Knowlton on Westerns

But climate change has brought an element of doubt into their future. For now, the caves are a regularly occurring feature, notably along the shoreline near Sault Ste Marie, Ontario. Made from snow and ice, the caves vary in size, shape and colour. Kris T December 22nd, Application , Product. General tips for western blot reproducibility. Reproducible immunoblot , Western blot troubleshooting. General tips for western blot reproducibility cwhoag T September 12th, Reproducible immunoblot , Western blot troubleshooting.

Read More. What went wrong? A Western Blot Troubleshooting Guide. Application , Product , Western blot troubleshooting. Want better westerns? Walk away! Before your experiment, you should verify that your antibodies are consistently specific and selective in that particular assay and context. In your experimental context, the primary antibody must recognize its intended target with minimal binding to other proteins in the sample.

Cross-reactivity also called off-target or non-specific binding occurs when the antibody recognizes other sample proteins with epitopes the portion of an antigen recognized by an antibody similar to the target protein. Selectivity and off-target binding are dependent on the relative abundance of target protein in your samples, as well as antibody concentration, dilution buffer, and incubation time.

Specificity, selectivity, and cross-reactivity should be assessed when you validate an antibody. Uhlen M. Nature Methods , 13, pp DOI: Combining several validation strategies is the most effective way to demonstrate antibody specificity and validate performance. Blocking buffers enhance the sensitivity and specificity of Western blotting by reducing non-specific binding of antibodies to the membrane surface and to off-target sample proteins.

Your goal is to block these non-specific sites without affecting detection of the target protein. But each antibody-antigen pair is unique — and the blocking buffer you choose can have a surprisingly strong influence on antibody specificity and performance.